Report error Found 3023 Enz. Inhib. hit(s) with Target = '5'-nucleotidase'
Affinity DataIC50: 0.00270nMAssay Description:Inhibition of CD73 in human CD8-positive T cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2....More data for this Ligand-Target Pair
Affinity DataKi: 0.00500nMAssay Description:Inhibition of recombinant human C-terminal His-tagged CD73 (27 to 549 residues) expressed in HEK293 cells using AMP as substrate preincubated for 1 h...More data for this Ligand-Target Pair
Affinity DataKi: 0.00500nMAssay Description:Inhibition of human CD73More data for this Ligand-Target Pair
Affinity DataKi: 0.00500nMAssay Description:Binding affinity to human recombinant CD73 assessed as inhibition constantMore data for this Ligand-Target Pair
Affinity DataKi: 0.00500nMAssay Description:Competitive reversible inhibition of human C-terminal His6-tagged CD73 expressed in HEK293 cells using AMP as substrate preincubated with substrate f...More data for this Ligand-Target Pair
Affinity DataIC50: 0.00800nMAssay Description:Inhibition of CD73 in human CD8-positive T cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2....More data for this Ligand-Target Pair
Affinity DataIC50: 0.00850nMAssay Description:Inhibition of CD73 in human PBMC using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2.5 hrs by PiColor...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0100nMAssay Description:Inhibition of CD73 in human CD8-positive T cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2....More data for this Ligand-Target Pair
Affinity DataIC50: 0.0110nMAssay Description:Inhibition of CD73 in human PBMC using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2.5 hrs by PiColor...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0130nMAssay Description:I. Experimental Materials and Equipment1. Malachite green phosphate detection kit (R & D Systems, Cat #, DY996)2. Recombinant human 5′-nucleoti...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0210nMAssay Description:Inhibition of CD73 in human PBMC using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 2.5 hrs by PiColor...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0270nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0280nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0310nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0310nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0320nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataKi: 0.0380nMAssay Description:Competitive inhibition of soluble C-terminal 6His-tagged recombinant human CD73 (27 to 547 residues) expressed in CHO cells assessed as inhibition co...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0410nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0430nMAssay Description:Inhibition of soluble human CD73More data for this Ligand-Target Pair
Affinity DataIC50: 0.0450nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0500nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0500nMAssay Description:Inhibition of human CD73 expressed in A-375 cells incubated for 15 mins by malachite green based spectrophotometer assayMore data for this Ligand-Target Pair
Affinity DataIC50: 0.0500nMAssay Description:Inhibition of human CD73More data for this Ligand-Target Pair
Affinity DataIC50: 0.0510nMAssay Description:Inhibition of human CD73 expressed in A-375 cellsMore data for this Ligand-Target Pair
Affinity DataIC50: 0.0520nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0530nMAssay Description:Inhibition of soluble human CD73More data for this Ligand-Target Pair
Affinity DataIC50: 0.0580nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0600nMAssay Description:Human recombinant protein CD73 (Acro, Cat. No. CD3-H52H7) was prepared into a 0.04 ng/μL protein solution, and substrate AMP (Sigma, Cat. No. A1752-1...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0600nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0700nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0740nMAssay Description:I. Experimental Materials and Equipment1. Malachite green phosphate detection kit (R & D Systems, Cat #, DY996)2. Recombinant human 5′-nucleoti...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0800nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.0950nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of CD73 in human plasma derived from esophageal cancer patient incubated for 15 mins in presence of 15N5-AMP by LC/MS analysisMore data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:Inhibition of human CA2More data for this Ligand-Target Pair
Affinity DataIC50: 0.100nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.110nMAssay Description:I. Experimental Materials and Equipment1. Malachite green phosphate detection kit (R & D Systems, Cat #, DY996)2. Recombinant human 5′-nucleoti...More data for this Ligand-Target Pair
Affinity DataKi: 0.110nMAssay Description:Competitive inhibition of cell surface anchored CD73 (unknown origin) expressed in human SK-MEL-28 cells assessed as inhibition constant incubated fo...More data for this Ligand-Target Pair
Affinity DataIC50: 0.110nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.115nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.120nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.130nMAssay Description:I. Experimental Materials and Equipment1. Malachite green phosphate detection kit (R & D Systems, Cat #, DY996)2. Recombinant human 5′-nucleoti...More data for this Ligand-Target Pair
Affinity DataIC50: 0.130nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.130nMAssay Description:Compound serial dilutions were pre-spotted into Thermo Nunc assay plate. 50 uL CD73 enzyme buffer (CD73 purchased from R&D system=0.6 nM, 25 mM Tris,...More data for this Ligand-Target Pair
Ligand InfoSimilars
Affinity DataIC50: 0.140nMAssay Description:For measurements of soluble CD73 enzyme activity, recombinant CD73 was obtained from R&D Systems, Cat. No. 5795-EN-010. Serial dilutions of test comp...More data for this Ligand-Target Pair
Affinity DataIC50: 0.140nMAssay Description:Compound serial dilutions were pre-spotted into Thermo Nunc assay plate. 50 uL CD73 enzyme buffer (CD73 purchased from R&D system=0.6 nM, 25 mM Tris,...More data for this Ligand-Target Pair
Ligand InfoSimilars
Affinity DataIC50: 0.150nMAssay Description:Compound serial dilutions were pre-spotted into Thermo Nunc assay plate. 50 uL CD73 enzyme buffer (CD73 purchased from R&D system=0.6 nM, 25 mM Tris,...More data for this Ligand-Target Pair
Ligand InfoSimilars
Affinity DataIC50: 0.150nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair
Affinity DataIC50: 0.150nMAssay Description:Compound serial dilutions were pre-spotted into Thermo Nunc assay plate. 50 uL CD73 enzyme buffer (CD73 purchased from R&D system=0.6 nM, 25 mM Tris,...More data for this Ligand-Target Pair
Ligand InfoSimilars
Affinity DataIC50: 0.150nMAssay Description:Inhibition of human CD73 expressed in CHO cells using AMP as substrate preincubated for 60 mins followed by substrate addition and measured after 60 ...More data for this Ligand-Target Pair


3D Structure (crystal)





























