Report error Found 4385 Enz. Inhib. hit(s) with Target = 'Muscarinic acetylcholine receptor M3'
Affinity DataEC50: 160nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 40nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 50nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 500nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 130nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 500nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 40nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 4.00E+3nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 79nMAssay Description:NIH-3T3 cells were grown in 96-well tissue culture plates to 70% to 80% confluence. Cells were transfected with plasmid DNAs using Superfect Reagent ...More data for this Ligand-Target Pair
Affinity DataEC50: 7.70E+4nMAssay Description:Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 mus...More data for this Ligand-Target Pair
Affinity DataEC50: 5.93E+4nMAssay Description:Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 mus...More data for this Ligand-Target Pair
Affinity DataEC50: 1.24E+4nMAssay Description:Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 mus...More data for this Ligand-Target Pair
Affinity DataEC50: 3.60E+5nMAssay Description:Assay Provider: P. Jeffrey Conn Assay Provider Affiliation: Vanderbilt University Grant Title: Discovery of novel allosteric modulators of the M1 mus...More data for this Ligand-Target Pair
Affinity DataEC50: 2.71E+3nMAssay Description:Selective M1 activation is an attractive therapeutic approach for the treatment of cognitive impairment, Alzheimer's disease, schizophrenia and a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.403nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,R176C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.484nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,A177C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.521nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,K178C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.499nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,R179C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.493nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T180C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.751nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.555nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,K548C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.555nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T549C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.564nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,F550C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.556nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,R551C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.524nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T552C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.438nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T553C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.548nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,F554C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.583nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,K555C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.553nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T556C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.462nMpH: 7.4Assay Description:In brief, binding reactions containing ~10 μg of membrane protein per tube were carried out for 2 h (22 °C) in 1 ml of binding buffer containing...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.440nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,R176C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.470nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T180C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 1.11nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,F550C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 1.15nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T553C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 1.02nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3 [1-273,470-589,N6Q,N15Q,N41Q,N48Q,N52Q,T556C](Rat)
National Institutes of Health
National Institutes of Health
Affinity DataKd: 0.640nMAssay Description:In brief, membranes from COS-7 cells expressing different Cys-substituted mutant M3R constructs were first treated with CuPhen (100 μM; 10 min a...More data for this Ligand-Target Pair
Affinity DataEC50: >1.00E+4nMAssay Description:Agonist activity at human muscarinic acetylcholine receptor M3 expressed in CHO cells assessed as increase in calcium mobilization incubated for 10 m...More data for this Ligand-Target Pair
Affinity DataEC50: 1.05E+4nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 1.00E+4nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 240nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 240nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 3.70E+3nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 5.43E+3nMAssay Description:Agonist activity at Gq/11 coupled recombinant human muscarinic M3 AChR expressed in CHO-FlpIn cells assessed as IP-one accumulation after 40 mins by ...More data for this Ligand-Target Pair
Affinity DataEC50: 23nMAssay Description:Agonist activity at recombinant human muscarinic M3 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins b...More data for this Ligand-Target Pair
Affinity DataEC50: 23nMAssay Description:Agonist activity at recombinant human muscarinic M3 receptor expressed in CHO cells assessed as upregulation in ERK1/2 phosphorylation after 5 mins b...More data for this Ligand-Target Pair
Affinity DataEC50: >3.00E+4nMAssay Description:Activity at human M3 receptorMore data for this Ligand-Target Pair
TargetMuscarinic acetylcholine receptor M3(Rat)
Vanderbilt University Medical Center
Curated by ChEMBL
Vanderbilt University Medical Center
Curated by ChEMBL
Affinity DataEC50: >3.00E+4nMAssay Description:Activity at rat M3 receptorMore data for this Ligand-Target Pair
Affinity DataEC50: >1.00E+4nMAssay Description:Positive allosteric modulation of human M3 receptor assessed as increase in acetylcholine-induced responseMore data for this Ligand-Target Pair
Affinity DataEC50: >3.00E+4nMAssay Description:Positive allosteric modulation of human M3 receptor expressed in CHO cells assessed as increase in acetylcholine-induced calcium mobilization pretrea...More data for this Ligand-Target Pair
Affinity DataEC50: >5.00E+4nMAssay Description:Contraction of guinea pig ileum by muscarinic AChR activation, which could be inhibited by application of atropineMore data for this Ligand-Target Pair














3D Structure (crystal)






