Reaction Details  Report a problem with these data
Cell Reactant:
Cytochrome P450 Reductase (CPR)
Syringe Reactant:
BDBM11942
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2006-11-07
ΔG°:
-39.2920±n/a (kJ/mole)
pH:
7.0000±n/a
Log10Kb:
6.1
Temperature:
298.1500±n/a (K)
ΔHobs :
-79.4200±8.3600 (kJ/mole)
Corrected for ΔHioniz:
not known
ΔCp :
-0.9196±n/a (kJ/mole)
Stoich. Param.:
1.0000
ΔS° :
-0.1318±n/a (kJ/mole-K)
Citation
 Hoffman, JM; Grunau, A; Smith, AM; Paine, MJ; Rooney, CS; Ladbury, JE; Fisher, TE; Gutierrez, A; Wai, JS; Thomas, CM; Bamberger, DL; Barnes, JL; Williams, TM; Jones, JH Global effects of the energetics of coenzyme binding: NADPH controls the protein interaction properties of human cytochrome P450 reductase. Biochemistry 45:1421-34 (2006) [PubMed]  Article
Cell React
Source:
Human fibroblast CPR (lacking the N-terminal membrane-anchoring region) and the functional FAD-binding domain were expressed in Escherichia coli BL21 (DE3).
Prep. Method:
The recombinant His-tagged proteins were purified to homogeneity by nickel-agarose chromatography. The notable exception is the omission of the 2,5-ADP affinity resin step to avoid the unusual biphasic binding isotherms during ITC experiment.
Name:
NADPH--cytochrome P450 reductase
Synonyms:
NADPH--cytochrome P450 reductase | P450R | NCPR_HUMAN | POR | CYPOR | Cytochrome P450 Reductase (CPR)
Type:
Enzyme
Mol. Mass.:
76675.22
Organism:
Human
Description:
P16435
Residue:
677
Sequence:
MGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEESSIRQYELVVHTDIDAAKVYMGEMGRLKSYENQKPPFDAKNPFLAAVTTNRKLNQGTERHLMHLELDISDSKIRYESGDHVAVYPANDSALVNQLGKILGADLDVVMSLNNLDEESNKKHPFPCPTSYRTALTYYLDITNPPRTNVLYELAQYASEPSEQELLRKMASSSGEGKELYLSWVVEARRHILAILQDCPSLRPPIDHLCELLPRLQARYYSIASSSKVHPNSVHICAVVVEYETKAGRINKGVATNWLRAKEPAGENGGRALVPMFVRKSQFRLPFKATTPVIMVGPGTGVAPFIGFIQERAWLRQQGKEVGETLLYYGCRRSDEDYLYREELAQFHRDGALTQLNVAFSREQSHKVYVQHLLKQDREHLWKLIEGGAHIYVCGDARNMARDVQNTFYDIVAELGAMEHAQAVDYIKKLMTKGRYSLDVWS
  
Syringe React
Source:
For H4NADP synthesis, High-purity hydrogen (99.995%) was supplied by BOC (Surrey, U.K.). Palladium (10% on activated charcoal) was purchased from ACROS Organics.
Prep. Method:
H4NADP was obtained by palladium-catalyzed hydrogenation of NADP(H).
Name:
BDBM11942
Synonyms:
1,4,5,6-tetrahydro-NADP | H4NADP
Type:
Nucleoside or nucleotide
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
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