Reaction Details 
Report a problem with these data
Report a problem with these dataCell Reactant:
Transcriptional Regulator TtgR
Syringe Reactant:
BDBM19461
Meas. Tech.:
Isothermal Titration Calorimetry
Entry Date.:
2008-07-23
ΔG°:
-27.5880±0.4180 (kJ/mole)
pH:
7.0000±n/a
Log10Kb:
3
Temperature:
303.1500±n/a (K)
ΔHobs :
-45.1440±0.4180 (kJ/mole)
Corrected for ΔHioniz:
no
ΔS° :
-0.0579±0.0014 (kJ/mole-K)
Citation
De Lucca, GV; Alguel, Y; Liang, J; Meng, C; De Lucca, I; Terán, W; Krell, T; Ramos, JL; Gallegos, MT; Zhang, X Crystal structures of multidrug binding protein TtgR in complex with antibiotics and plant antimicrobials. J Mol Biol 369:829-40 (2007) [PubMed] ArticleCell React
Source:
A 651-bp fragment containing the ttgR gene was amplified by PCR from P. putida DOT-T1E chromosomal DNA, and cloned in E. coli expression vector. TtgR protein was over-expressed and purified.
Prep. Method:
TtgR protein was further purified by size exclusion chromatography using a Sephacryl HR-200 column (Amersham Biosciences). Eluted fractions of TtgR were pooled, concentrated, and dialyzed against the buffer.
Name:
Transcriptional Regulator TtgR
Synonyms:
HTH-type transcriptional regulator ttgR | Toluene efflux pump ttgABC operon repressor | TTGR_PSEPT | ttgR
Type:
Repressor; homodimer
Mol. Mass.:
23852.57
Organism:
Pseudomonas putida
Description:
n/a
Residue:
210
Sequence:
MVRRTKEEAQETRAQIIEAAERAFYKRGVARTTLADIAELAGVTRGAIYWHFNNKAELVQALLDSLHETHDHLARASESEDEVDPLGCMRKLLLQVFNELVLDARTRRINEILHHKCEFTDDMCEIRQQRQSAVLDCHKGITLALANAVRRGQLPGELDAERAAVAMFAYVDGLIRRWLLLPDSVDLLGDVEKWVDTGLDMLRLSPALRK
Syringe React
Name:
BDBM19461
Synonyms:
5,7-dihydroxy-2-(4-hydroxyphenyl)-3,4-dihydro-2H-1-benzopyran-4-one | CHEMBL32571 | naringetol | Naringenin | salipurpol | Naringenin (NAR) | α-CA inhibitor, 5
Type:
Estrgen Antagonist
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
