Reaction Details  Report a problem with these data
Cell Reactant:
Thymidine Kinase
Syringe Reactant:
BDBM3
Meas. Tech.:
ITC
Entry Date.:
2000-12-13
ΔG°:
-16.89±0.35 (kcal/mole)
pH:
7.5000±n/a
Temperature:
298.1500±n/a (K)
ΔH° :
-33.26±1.66 (kJ/mole)
Corrected for ΔHioniz:
yes
Protons Released:
-0.3500
ΔCp :
-0.51±0.02 (kJ/mole)
ΔS° :
-0.06±0.01 (kJ/mole-K)
Citation
 Perozzo, R; Jelesarov, I; Bosshard, HR; Folkers, G; Scapozza, L Compulsory order of substrate binding to herpes simplex virus type 1 thymidine kinase. A calorimetric study. J Biol Chem 275:16139-45 (2000) [PubMed]  Article
Cell React
Source:
Expressed as glutathione S-transferase fusion protein in E. coli.
Prep. Method:
Purified by glutathione affinity chromatography.
Name:
Thymidine kinase
Synonyms:
KITH_HHV11 | TK
Type:
Enzyme
Mol. Mass.:
40978.57
Organism:
HHV-1
Description:
P0DTH5
Residue:
376
Sequence:
MASYPCHQHASAFDQAARSRGHNNRRTALRPRRQQKATEVRLEQKMPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN
  
Syringe React
Source:
Sigma
Name:
BDBM3
Synonyms:
thymidine/ATP (1:1 mixture)
Type:
n/a
Emp. Form.:
TBD
Mol. Mass.:
TBD
SMILES:
TBD
Structure:
Search PDB for entries with ligand similarity: