| Assay Method Information | |
| | Homogeneous Time-resolved Fluorescence (HTRF) Assay |
| Description: | The assay uses purified enzyme interacting with biotinylated peptide substrate. HTRF is based on the proximity of europium cryptate (donor fluorophore)-labeled antiphosphotyrosine and streptavidin labeled with APC(allophycocyanin), which have been brought together by a binding reaction. When the two entities come into close proximity and upon excitation, energy transfer occurs and APC re-emits at 665 nm. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |