| Assay Method Information | |
| | PRMT5 Enzyme Inhibitory Activity Assay |
| Description: | Experimental objective: To test the inhibitory effect of compounds on PRMT5 enzyme activity. Experimental materials: PRMT5 enzyme. Experimental operation: Test compounds were added at a certain concentration (0.17 nM to 99010 nM) to a white transparent bottomed 384-well plate using LABCYTE Echo 550, then PRMT5 was added, and a vehicle control (with DMSO and without compound) and a blank control (with DMSO and without PRMT5) were set up. The plate was incubated at 25° C. for 30 min, then added with substrate, and reacted at 25° C. for 90 min. After 90 min, the detection reagent was added to the 384-well plate using PerkinElmer LANCE Ultra TR-FRET standard method to combine with the enzyme-substrate reaction product. After 1 hour of reaction at 25° C., the signal was detected on a PerkinElmer EnVision 2105 Multimode Plate Reader. |
| Affinity data for this assay | |
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