| Assay Method Information | |
| | In Vitro Assay |
| Description: | Preparation of working solution: 2×Glutaminyl Cyclase substrate was diluted in assay buffer to 5 M. 2×enzyme solution and QPCTL was diluted in assay buffer to 0.8 nM. Glutaminyl Cyclase Developer was diluted in assay buffer to 1×(containing 10 mM 1-Benzyl-Imidazole). Serial dilution of test compounds was performed in DMSO. The final DMSO concentration in 100 μl reaction was 1%. The assay buffer was 50 mM Tris, pH 8.0, 0.01% BSA.Procedure: 50 μL of 2×Glutaminyl Cyclase substrate solution was added into the assay plate followed by 50 μL of 2×enzyme solution. The plate was centrifuged for 1 min at 1000 rmp. Next the plate was incubated for 45 minutes at 37° C. This was followed by the additional of 50 μL of the prepared Glutaminyl Cyclase developer and incubated for additional 30 min at 37° C. Results were read on SpectraMax Paradigm detecting with emission at Ex/Em=490 nm/520 nm. |
| Affinity data for this assay | |
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