| Assay Method Information | |
| | Potential Inhibitory Effect on Voltage-Gated Potassium Channel hERG by Fluorescence Polarization Technique |
| Description: | In this experiment, IC50 was calculated by detecting the effect of compounds at 8 concentrations on the current of the hERG channel. The procedures were performed exactly as for Predictor hERG fluorescence polarization assay kit. Before measuring fluorescence polarization, the assay plate was covered to protect the reagents from light and evaporation, and after incubation at room temperature for 2-4 h, the sample plate was centrifuged to read the fluorescence polarization value, with the excitation light at 540 nm and the emission light at 573 nm. The data showed that the cardiotoxicity of the compounds of the present disclosure was significantly lower than that of the marketed drug midazolam. |
| Affinity data for this assay | |
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