| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | HIV-1 protease activity was measured by a continuous fluorometric assay using the internally quenched fluorogenic substrate DABCYL-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS. The proteolytic activity can be monitored by the increase in fluorescence intensity, and the protease products were analyzed by HPLC. |
| Affinity data for this assay | |
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