| Assay Method Information | |
| | DPP4 activity assay |
| Description: | Human DPP4 activity assay data were obtained using a DPP4 Activity Assay Kit (Sigma-Aldrich, MAK088) according to the manufacturer's instructions. Briefly, 10 μL of DPP4 Assay Buffer was transferred per well in low volume 384-well plates before transferring 10 μL of diluted compound dissolved in DPP4 assay buffer. 5 μL of Master Reaction Mix containing a fluorescent substrate that becomes fluorescent upon cleavage by the enzyme. Fluorescence intensity measurements were recorded at 1-minute time intervals over the course of 20 minutes using an Envision Multimode Plate Reader (PerkinElmer). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |