| Assay Method Information | |
| | Assay of Inhibitory Activity of Compounds of the Present Invention Against Wee-1 Kinase |
| Description: | After the serially diluted compound and an enzyme were mixed, the mixture was incubated at room temperature (25° C.) for 15 min, and then centrifuged at 1000 rpm for 1 min for mixing. 5 μL of substrate was added to start the reaction. After the mixture was reacted at room temperature for 60 min, 5 μL of ADP-GLO reagent was added and the mixture was centrifuged at 1000 rpm for 1 min for mixing. The mixture was subsequently incubated at room temperature for 60 min, and then 10 μL of kinase detection reagent was added. The mixture was incubated for 60 min, and the chemiluminescence was determined. |
| Affinity data for this assay | |
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