| Assay Method Information | |
| | sAC Biochemical Cyclase Assay |
| Description: | Assays for sAC activity using purified protein were performed in 100 ul reactions containing 4 mM MgCl2, 2 mM CaCl2, 1 mM ATP, 40 mM NaHCO3, 50 mM Tris pH 7.5, and 3 mM DTT. Each reaction contained 1,000,000 counts of alpha-32P labeled ATP. Generated cAMP was purified using sequential Dowex and Alumina chromatography. |
| Affinity data for this assay | |
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