| Assay Method Information | |
| | AXL Kinase Inhibitory Activity Assay |
| Description: | AXL enzyme (Carna, 08-107) configuration and addition: 33.33 ng/μL of AXL enzyme was diluted to 0.027 ng/μL (1.67×, final conc.=0.016 ng/μL) with 1× enzyme buffer (1 ml of 1× enzyme buffer configured with 200 μL of Enzymatic buffer kinase 5×, 10 μL of 500 mM MgCl2, 10 μL of 100 mM DTT, and 6.26 μL of 2500 nM SEB, with the addition of 773.75 μL of H2O), using a BioTek (MultiFloFX) automated dispenser, compound wells and positive control wells were each spiked with 6 μL of 1.67 times the final concentration of enzyme solution; 6 μL of 1× Enzymatic buffer was added to the negative control wells. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |