| Assay Method Information | |
| | V1b Competitive Radioligand Binding Assay |
| Description: | Test compounds were tested for binding to the V1b human vasopressin receptor using a human V1b (agonist radioligand) Receptor Binding Assay, with the results summarized in Table 2. Test compounds were tested at several concentrations for IC50 or EC50 determination.The detection method used was Scintillation counting. Ligand [3H]AVP was used with a Kd of 0.2 nM at concentrations of 0.2 nM. Incubation was for 120 min at RT. Control inhibitor used was Argipressin acetate and reference compound was AVP.Compound binding was calculated as a % inhibition of the binding of a ligand specific for each target. The results are expressed as a percent of control specific bindingmeasuredspecificbindingcontrolspecificbinding*100and as a percent inhibition of control specific binding100-(measuredspecificbindingcontrolspecificbinding*100)obtained in the presence of the test compounds. |
| Affinity data for this assay | |
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