| Assay Method Information | |
| | Homogenouse Time-Resolved Fluorescence (HTRF) Binding Assay |
| Description: | The purchased kit (CisBio, #64CUS000C-1) contains reagents required for experiments such as PD-1, PD-L1, anti-tag1-Eu, Anti-tag2-XL665, Dilute Buffer, and Detection Buffer.Experimental Steps1. The compound was configured with 100% DMSO into 10 concentrations with a concentration gradient of 3 times.2. The DMSO solution of the compound was added to Dilute Buffer solution, and the mixture was mixed well and transferred to a 96-well plate.3. PD-L1 was diluted with Dilute Buffer solution and then added to the above 96-well plate.4. PD-1 was diluted with Dilute Buffer solution and then added to the above 96-well plate, and incubated at room temperature for 30 minutes.5. One part of anti-tag1-Eu and one part of Anti-tag2-XL665 were added to Detection Buffer solution, and the mixture was mixed well and transferred to the above 96-well plate.6. The mixture in the 96-well plate was incubated at room temperature for 1 to 24 hours.7. The HTRF values were read with Envision. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |