| Assay Method Information | |
| | SPR Analysis |
| Description: | The interaction of PSMA-binding variants with human FOLH1 was measured with Surface Plasmon Resonance (SPR) technology. Biotinylated FOLH1 was captured at a concentration of 30 μg/mL onto a Streptavidin chip. Binding kinetics of the analytes were measured with a Biacore 8K instrument in two-fold serial dilutions starting at 500 nM down to 1.95 nM in single cycle kinetic measurements. After each cycle, needles were washed with 50% DMSO. To measure the association to the FOLH1 protein, the samples were injected with a flow rate of 30 μL/min for 90 s, followed by 1200 s buffer only to detect the dissociation. The used running buffer was 1×PBS with 0.05% Tween20 and 2% DMSO. The relative response units (RU, Y-axis) are plotted against time (s, X-axis) and analyzed in a kinetic 1:1 binding model. In a different setup, biotinylated FOLH1 was captured at a concentration of 0.8 μg/mL and the running buffer was 1×PBS with 0.05% Tween20. |
| Affinity data for this assay | |
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