| Assay Method Information | |
| | Enzymatic activity assay |
| Description: | HDAC proteins of different subtypes (purchased from BPS), test compounds, and substrate solutions at concentrations of 2.5-40 M (purchased from BPS) were each added to a 384-well plate, and the plate was incubated at 37° C. for 30 min. 1 M Trypsin and 10 M TSA were added, and the plate was incubated at room temperature for 15 min. The fluorescence intensity at an excitation wavelength of 360 nm and an emission wavelength of 455 nm was measured, and then the inhibition rate at each concentration was calculated. IC50 was obtained by fitting using GraphPadPrism 7.0 software. |
| Affinity data for this assay | |
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