| Assay Method Information | |
| | Assessment of HIF-1α and 2α Binding Activity in In Vitro Assays |
| Description: | The binding affinity of the tripeptides was determined against the recombinantly expressed PAS-B domain of both HIF-1α and 2α using microscale thermophoresis (MST). HIF-α proteins were labelled with Monolith NT-647 labelling dye (Nanotemper Technologies GmbH) according to the manufacturer's instructions. MST experiments were performed on a Monolith NT.115 system (Nanotemper Technologies GmbH), in assay buffer containing 50 nM labelled protein, 10% DMSO and 0.05% TWEEN-20. MST measurements were performed using 50% LED and 50% MST power. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |