| Assay Method Information | |
| | ELISA assay for specific human IL-23R inhibitors |
| Description: | A 384-well plate was coated with 50 pl/well of human IL-23 at a final concentration of 1 pg/ml and incubated overnight at 4°C. The wells were washed 3 times with 80 pl wash buffer and blocked with 80 pl blocking buffer for 60 minutes at room temperature and washed again. 20 pl of serially diluted test peptides was added to each well and subsequently 20 pl of recombinant human IL-23R-Fc chimera at final concentration of 0.1 pg/ml. The plate was incubated for 60 minutes at room temperature. After the wells were washed, bound IL-23R-Fc was detected with goat anti-hu- IgG 1 -HRP antibody. Signals were visualized with QuantaBlu Fluorogenic Peroxidase Substrate. |
| Affinity data for this assay | |
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