| Assay Method Information | |
| | CDK Inhibition Assay |
| Description: | In vitro kinase assay using purified CDK enzyme was incubated at room temperature with substrate, and test compounds in the presence of 100 uM ATP/ [gamma-33P] ATP. Assays were performed in 96-well polypropylene plates. 33P incorporation into substrate was measured using a scintillation counter. |
| Affinity data for this assay | |
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