| Assay Method Information | |
| | Caspase Inhibition Assay |
| Description: | The substrate peptides terminating in AMC are processed by caspases with or without inhibitors, and the accumulation of AMC was assessed with a Cytofluor fluorescent plate reader at excitation and emission wavelengths of 380 and 460 nm. Compounds were tested, and full IC50 curves were run for all compounds expressing measurable inhibitory activity. |
| Affinity data for this assay | |
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