| Assay Method Information | |
| | Phosphatase Inhibition Assay |
| Description: | The activity of PTP1B enzyme was assayed with 4-nitrophenyl phosphate (pNPP) as substrate. Rate of formation of the phenolate ion was monitored at 410 nm on a spectramax 384 plate reader. Slopes of initial reaction rates (15 minute reactions) were plotted and fit to the Michaelis-Mention equation by nonlinear regression analysis (Graphpad Prism). |
| Affinity data for this assay | |
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