| Assay Method Information | |
| | In Vitro Enzyme Assay of FPT |
| Description: | FPT activity was determined by measuring transfer of [3H] farnesyl from [3H]farnesyl pyrophosphate to the substrate His6-Ha-Ras-CVLS. The incorporated radioactivity was measured in a Wallac 1204 liquid scintillation counter. Percent inhibition was calculated relative to the DMSO vehicle control. |
| Affinity data for this assay | |
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