| Assay Method Information | |
| | Akt Inhibition Assay |
| Description: | Purified recombinant human Akt1, Akt2, and Akt3 proteins were monitored for kinase activity in the presence or absence of Akt inhibitors in a 96-well format. Detection was done by homogeneous time-resolved fluorescence (HTRF) using a europium-labeled Phospho (S21)-GSK3a antibody, and streptavidin-linked XL665 fluorophore which binds to the biotin moiety on the substrate peptide. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |