| Assay Method Information | |
| | Enzyme Inhibition Measurements |
| Description: | Enzyme activities were assayed by monitoring the hydrolysis of substrate in the presence or absence of inhibitor compounds. The hydrolysis was recorded as the increase in fluorescence intensity over a 10 min time period. IC50 values were obtained by assuming competitive inhibition and fitting the dose response data to the equation (Vi/V0) =1/ (1 + [I]/IC50)). The Ki was calculated by using Ki = IC50/ (1 + [S]/Km), and a Km value determined according to Michaelis-Menten. |
| Affinity data for this assay | |
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