| Assay Method Information | |
| | RAF Kinase Assay |
| Description: | The biochemical activity of compound was determined by incubation with Raf kinases and substrate MEK-1 in the presence of 1-10 uM ATP/ [gamma-32P] ATP. After incubation, the reaction was stopped and the phosphorylated MEK-1 was harvested by filtration, and filter- bound radioactivity was quantified by beta-plate counter. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |