| Assay Method Information | |
| | Competition-Based Ligand Binding Assay. |
| Description: | Ligand binding was determined using a scintillation proximity assay with streptavidin-coated polyvinyltoluene scintillation beads (Amersham) and biotinylated receptor. Receptor-bound [3H]-E2 was determined by scintillation counting (Perkin-Elmer). The IC50 values were calculated using a four-parameter logistic equation. |
| Affinity data for this assay | |
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