| Assay Method Information | |
| | alpha-Glucosidase Inhibition Assay |
| Description: | The alpha-glucosidase inhibitory activity of test compounds was determined in a 96-well plate format. The reaction mixture containing enzyme and chromogenic substrate in the presence or absence of test compounds were incubated, and the amount of released p-nitrophenol was measured based on the absorbance at 405 nm. The IC50 values were calculated from the dose-response curves. |
| Affinity data for this assay | |
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