| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | The IC50 inhibition assays were performed in a 96-well microtiter plate format using purified recombinant IDO, which was added to the substrate, L-tryptophan (L-Trp), and the inhibitor. After the reaction, the product N-formylkynurenine was converted to kynurenine. The yellow color generated from reaction with kynurenine was measured at 490 nm using a microtiter plate reader. The data were analyzed using Graph Pad Prism 4 software (Graph Pad Software Inc.). |
| Affinity data for this assay | |
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