| Assay Method Information | |
| | Solid-Phase ELISA Kinase Assay |
| Description: | The 96-well flat-bottomed plates were coated with recombinant GST-BAD. After the plates were blocked, the reaction buffer containing test compound and recombinant GST-PIM1 kinase were added to each well. Phosphorylated GST-BAD was detected by an ELISA reaction. The level of phosphorylated GST-BAD present was proportional to the absorbance at 450 nm. Curve fitting and enzyme analyses were done using GraphPad Prism version 4.00 (GraphPad Software, San Diego, CA). |
| Affinity data for this assay | |
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