| Assay Method Information | |
| | Aurora Kinase Assay |
| Description: | Assays for Aurora kinase was performed in a DELFIA format. Aurora enzyme was incubated with test compound and cross-tide substrate in the reaction buffer. After incubation, the reaction mixtures were transferred to a neutravidin-coated plate, and phosphorylated peptide was quantified by means of a phosphor-specific antibody (Cell signaling Technology) and a europium labeled secondary antibody (Perkin-Elmer) using time-resolved fluorescence (excitation, 337 nm; emission, 620 nm). |
| Affinity data for this assay | |
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