| Assay Method Information | |
| | PARP ELISA Assay |
| Description: | The potency of inhibition on purified PARP enzyme was determined for selected compounds, and the potency was compared with that of 3-aminobenzamide (3-AB), a prototypical benchmark PARP inhibitor. The assay was performed in 96-well ELISA plates according to instructions provided with a commercially available PARP inhibition assay kit (Trevigen, Gaithersburg, MD). After incubation, incorporated biotin was detected by peroxidase-conjugated streptavidin and TACS Sapphire substrate. |
| Affinity data for this assay | |
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