| Assay Method Information | |
| | Radioligand Binding Assay (IC50) and Cell-Based Transcription Assay (EC50) |
| Description: | Competition-binding curves for test compounds were determined with expressed human PPAR LBD. Plots of inhibitor concentration versus cpm of radioligand bound were constructed. IC50 values were obtained from a nonlinear least squares fit of the data. EC50 is the concentration of test compounds needed to induce 50% of the maximum alkaline phosphatase activity in CV-1 cells transfected with PPAR and a GAL4 reporter construct expressing secreted placental alkaline phosphatase (SPAP). |
| Affinity data for this assay | |
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