| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | The in vitro anti-fXa activity was measured by using a chromogenic substrate S-2222 and human fXa. Aqueous DMSO or test compounds in aqueous DMSO and human fXa were mixed with reaction buffer. A reaction was started by the addition of substrate S-2222. The absorbance (OD) at 405 nm was monitored every 10 s with a SPECTRAmax 340 microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) at room temperature and the reaction velocity (OD/min) was obtained. The anti-fXa activity (inhibition %) was then calculated. The IC50 value was obtained by plotting the compound concentration against the anti-fXa activity. |
| Affinity data for this assay | |
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