| Assay Method Information | |
| | Tyrosine Kinase Assay |
| Description: | The assays were performed in 96-well microtiter plates that had been coated with a polyGluTyr peptide. Negative control wells received buffer alone without ATP. IC50 is the inhibitor concentration which inhibits 50% of kinase activity that catalyzes the transfer of the terminal phosphate to the immobilized substrate. The amount of phosphotyrosine generated was quantitated by using anti-posphotyrosine antibody. |
| Affinity data for this assay | |
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