| Assay Method Information | |
| | Protease Inhibition Assay |
| Description: | The inhibitory activities of the compounds toward HIV-1 PR were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) assay. The Ki values of active compounds were determined using the partially purified protease and the synthetic substrate Ac-Ser-Gln-AsnTyr-Pro-Ile-Val-NH2. |
| Affinity data for this assay | |
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