| Assay Method Information | |
| | alpha-Glucosidase Assay |
| Description: | 10 µL of test samples (5 mg/mL DMSO solution) were reconstituted in 100 µL of 100 mM-phosphate buffer (pH6.8) in 96-well microplate and incubated with 50 µL ofcrude intestinal α-glucosidase for 5 min before 50 µL substrate (5 mM, p-nitrophenyl-α-D-glucopyranoside prepared in same buffer) was added. Release of p-nitrophenol was measured at 405 nm spectrophotometrically for 5 min after incubation with substrate. Individual blanks for test samples were prepared to correct background absorbance where substrate was replaced with 50 µL of buffer. Control sample contained 10 µL DMSO in place of test samples |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |