| Assay Method Information | |
| | Kinase-Glo Luminescent Kinase Assay |
| Description: | Briefly, recombinant human GSK3β (1 µM, 10 µL) was mixed with 10 µL of GSK3β substrate (YRRAAVPPSPSLSRHSSPHQpSEDEEE, 100 µM), 19 µL of 2.5 × kinase buffer (50 mM Tris-HCl, pH 7.4, 25 mM MgCl2), 10 µL of 10 µM ATP, 1 µL of the indicated amount of chemicals in DMSO to a final volume of 50 µL. Control reaction without kinase was set up. The mixtures were incubated at 30 °C for 40 min. The enzymatic reaction was stopped with 50 µL of Kinase-Glo reagent. Glow-type luminescence was recorded after 10 min. |
| Affinity data for this assay | |
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