| Assay Method Information | |
| | iNOS Activity Assay |
| Description: | The iNOS activity assay was based on the method of hemoglobin assay previously described with slight modifications. The activity of the enzyme was determined spectrophotometrically by monitoring the NO-mediated conversion of oxyhemoglobin to methemoglobin at 401 and 421 nm. |
| Affinity data for this assay | |
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