| Assay Method Information | |
| | Thymidine Phosphorylase Inhibition Assay |
| Description: | TP inhibition assay was performed spectrophotometrically. The method of Bera et al. was followed with slight modifications. Reaction mixture of 200 µL, contained 20 µL of enzyme (0.058 unit/well), 150 µL of potassium phosphate buffer (pH 7.0, 50 mM), and 10 µL of test compound (0.5 mM). The reaction mixture was then incubated for 10 min at 30 °C. After 10 min, substrate (thymidine, kmax; 265 nm) (20 µL, 1.5 mM) was added, and change in absorbance was observed for 10 min at 290 nm in 96-well ELISA plate reader (Spectramax, Molecular Devices, CA, USA). Every experiment was run in triplicate. 7-Deazaxanthine was used as positive control. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |