| Assay Method Information | |
| | Proteolytic Activity Assay |
| Description: | The proteolytic activity was estimated at pH 5.0, 37 °C using 0.1 M acetate buffer as the incubation medium. The homogenate prepared above was incubated with the buffer at 37 °C for 3 h and 24 h (Fig. 1), separately. The reaction was stopped by the addition of TCA and the resulting solution was centrifuged to precipitate proteins. The acid soluble proteins were quantitated in the supernatant using Bradford method [Anal. Biochem., 72:248-254]. |
| Affinity data for this assay | |
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