| Assay Method Information | |
| | Test for beta-lactamase Inhibitory Activity |
| Description: | he inhibitory activities of present compounds against various enzymes were measured by spectrophotometric assay using 490 nM and using nitrocefin as a substrate [J. Antimicrob. Chemother., 28, pp 775-776 (1991)]. The concentration of inhibitor (IC50) which inhibits by 50% the reaction of hydrolysis of nitrocefin by the enzyme is determined. |
| Affinity data for this assay | |
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