| Assay Method Information | |
| | CYP19 assay |
| Description: | The enzyme activity was assayed by measuring the 3H-labeled H2O formed from [1beta-3H] Androstenedione during aromatization. After incubation, the reaction was terminated, and the charcoal-adsorbed steroids were separated by centrifugation. Aliquots of the supernatant were assayed for the presence of 3H2O by counting using a liquid scintillation spectrometer |
| Affinity data for this assay | |
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