| Assay Method Information | |
| | Affinity Test Between the Compounds of the Invention and hPD-L1 Protein |
| Description: | The test compound was dissolved in DMSO to make a stock solution with a final concentration of 10 mM, then diluted to 1× with 1.05× PBS-P+, and then diluted by 4 times with 1× PBS-P+ buffer containing 5% DMSO; the Fc-PD-L1 protein was diluted to 10 μg/mL with 1× PBS-P+ solution containing 5% DMSO, and was then captured on the Protein A chip at a flow rate of 10 μl/min for 120 s. Each experiment used dual channels, one of which captured the ligand and the other served as a reference channel; the analyte was ready for use, and at least five concentrations were set for each analyte. The concentration selection varied with the analyte. The analyte flowed through the two channels at 30 μl/min for 90 seconds; solvent calibration was used for exclusion of the influence of DMSO, 1× PBS-P+ in 50% DMSO solution was used to wash the flow path with 30 μl/min; 10 mM glycine pH 1.5 for 30 s was used for chip surface regeneration, the experimental temperature is 25° C.; Biacore T200 Evaluation software was used, 1:1 binding model was used to calculate the KD value. |
| Affinity data for this assay | |
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