Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The kinase activity was assayed by the autophosphorylation reaction of ALK5 (T204D) in the presence of 4 uM ATP and [33P]-gamma-ATP. After incubation, phosphorylated receptors were captured and scintillation counted.
Affinity data for this assay
 

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