| Assay Method Information | |
| | Mouse and Rat 11beta-HSD1 SPA Assay |
| Description: | Enzyme assays were performed using purified recombinant enzymes, incubating with test compound and [3H]cortisone/NADPH. After incubation, the amount of the product, [3H]-cortisol, captured on the anticortisol antibody-coated SPA beads was determined in a microplate liquid scintillation counter. Kinetic constants were calculated using the Microsoft Excel integrated application XLfit (Version 5.3.0.19, ID Business Solutions Ltd.) using the sigmoidal dose-response model 205, which is based on the nonlinear curve fitting based on Levenberg-Marquardts algorithm. |
| Affinity data for this assay | |
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