| Assay Method Information | |
| | 11beta-HSD2 Homogeneous Time-Resolved Fluorescence (HTRF) Assay |
| Description: | 11beta-HSD2 enzyme activity was assessed in buffer containing NAD, test compound, and human recombinant 11beta-HSD2 expressed in E. coli. Reaction was initiated by addition of cortisol and terminated with addition of carbenoxolone stop solution after 60 min incubation at room temperature. Determinations of remaining cortisol levels were monitored by HTRF assay (Cisbio International). |
| Affinity data for this assay | |
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