Assay Method Information

Assay Name:  ChEMBL_2364624
Description:  Competitive binding affinity to 6His-tagged BRD4 BD1 (unknown origin) transfected in Escherichia coli BL21(DE3)-R3-pRARE2 cells incubated for 30 mins in presence of AlexaFluor647 dye conjugated tert-butyl (S)-2-(4-(4-chlorophenyl)-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl)acetate by TR-FRET assay
Affinity data for this assay
 

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