| Assay Method Information | |
| | Measurement of Dissociation Constants (Kd) by ITC |
| Description: | ITC experiments were performed using a Nano ITC isothermal titration calorimeter from TA Instruments. Experiments were performed in reverse-mode by titrating 50 μL of protein solution at a concentration of 300-600 μM into 350 μL of ligand solution at concentrations between 10-50 μM. Stir rate was 200-250 rpm with 16-25 injections, each at 2-3 μL with 150 seconds between each injection. Data fitting was performed using Nano ITCRun software. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |